Escherichia coli OP50
E396233
Escherichia coli OP50 is a uracil-requiring laboratory E. coli strain commonly used as the standard bacterial food source for Caenorhabditis elegans in research.
All labels observed (2)
| Label | Occurrences |
|---|---|
| E. coli OP50 | 1 |
| Escherichia coli OP50 canonical | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T3872107 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: Escherichia coli OP50 Context triple: [Caenorhabditis elegans, standardLabFood, Escherichia coli OP50]
-
A.
Caenorhabditis elegans
Caenorhabditis elegans is a small, transparent roundworm widely used as a model organism in biology and genetics due to its simple anatomy, fully mapped cell lineage, and well-characterized nervous system.
-
B.
Neurospora crassa
Neurospora crassa is a filamentous red bread mold fungus widely used as a model organism in genetics and molecular biology.
-
C.
Bacillus
Bacillus is a genus of rod-shaped, endospore-forming bacteria that includes both harmless environmental species and notable pathogens such as Bacillus anthracis.
-
D.
Saccharomyces cerevisiae
Saccharomyces cerevisiae is a species of yeast widely used in baking, brewing, and scientific research as a model eukaryotic organism.
-
E.
Drosophila melanogaster
Drosophila melanogaster is a small fruit fly species widely used as a model organism in genetics and developmental biology research.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: Escherichia coli OP50 Target entity description: Escherichia coli OP50 is a uracil-requiring laboratory E. coli strain commonly used as the standard bacterial food source for Caenorhabditis elegans in research.
-
A.
Caenorhabditis elegans
Caenorhabditis elegans is a small, transparent roundworm widely used as a model organism in biology and genetics due to its simple anatomy, fully mapped cell lineage, and well-characterized nervous system.
-
B.
Neurospora crassa
Neurospora crassa is a filamentous red bread mold fungus widely used as a model organism in genetics and molecular biology.
-
C.
Bacillus
Bacillus is a genus of rod-shaped, endospore-forming bacteria that includes both harmless environmental species and notable pathogens such as Bacillus anthracis.
-
D.
Saccharomyces cerevisiae
Saccharomyces cerevisiae is a species of yeast widely used in baking, brewing, and scientific research as a model eukaryotic organism.
-
E.
Drosophila melanogaster
Drosophila melanogaster is a small fruit fly species widely used as a model organism in genetics and developmental biology research.
- F. None of above. chosen
Statements (47)
| Predicate | Object |
|---|---|
| instanceOf |
Escherichia coli strain
ⓘ
bacterial strain ⓘ laboratory strain ⓘ |
| advantage |
thin lawn facilitates observation of Caenorhabditis elegans
ⓘ
uracil auxotrophy limits overgrowth on plates ⓘ |
| auxotrophyFor | uracil ⓘ |
| biosafetyLevel | BSL-1 ⓘ |
| cellType | prokaryote ⓘ |
| cellWall | outer membrane containing lipopolysaccharide ⓘ |
| culturedOn | nematode growth medium agar ⓘ |
| domain | Bacteria ⓘ |
| family |
Enterobacteriaceae
ⓘ
linked to:
Enterobacterales
|
| genus | Escherichia ⓘ |
| gramStain | Gram-negative ⓘ |
| growthCharacteristic | forms thin bacterial lawn on agar plates ⓘ |
| hasCommonName |
E. coli OP50
ⓘ
linked to:
Escherichia coli OP50
|
| isNonPathogenicFor |
Caenorhabditis elegans
ⓘ
healthy laboratory personnel ⓘ |
| metabolism | facultative anaerobe ⓘ |
| morphology | rod-shaped bacterium ⓘ |
| nutritionalRequirement | uracil-requiring ⓘ |
| organismFed |
Caenorhabditis briggsae
ⓘ
Caenorhabditis elegans ⓘ |
| organismPartOf | C. elegans standard laboratory diet ⓘ |
| oxygenRequirement |
can grow aerobically
ⓘ
can grow anaerobically ⓘ |
| parentTaxon | Escherichia coli ⓘ |
| reproduction | binary fission ⓘ |
| roleInExperiment |
food source
ⓘ
lawn-forming bacterium on nematode growth medium ⓘ |
| storageCondition | frozen glycerol stocks at −80 °C ⓘ |
| taxonRank | strain ⓘ |
| typicalIncubationTemperature | 37 °C ⓘ |
| usedAs |
bacterial food source in C. elegans research
ⓘ
standard food source for Caenorhabditis elegans ⓘ |
| usedAsControlIn |
dietary intervention studies in C. elegans
ⓘ
microbiome manipulation experiments in C. elegans ⓘ |
| usedBy |
C. elegans research community
ⓘ
model organism laboratories ⓘ |
| usedFor |
maintenance of C. elegans stocks
ⓘ
synchronization of C. elegans populations ⓘ |
| usedIn |
Caenorhabditis elegans behavior assays
ⓘ
Caenorhabditis elegans developmental studies ⓘ Caenorhabditis elegans genetics research ⓘ Caenorhabditis elegans laboratory culture ⓘ Caenorhabditis elegans lifespan studies ⓘ Caenorhabditis elegans maintenance on agar plates ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: Escherichia coli OP50 Description of subject: Escherichia coli OP50 is a uracil-requiring laboratory E. coli strain commonly used as the standard bacterial food source for Caenorhabditis elegans in research.
Referenced by (2)
Full triples — surface form annotated when it differs from this entity's canonical label.
linked to: Escherichia coli OP50