DNA ligase
E261823
DNA ligase is an essential enzyme that joins breaks in the sugar-phosphate backbone of DNA, enabling processes such as DNA replication and genetic recombination.
All labels observed (1)
| Label | Occurrences |
|---|---|
| DNA ligase canonical | 2 |
How this entity was disambiguated
This entity first appeared as the object of triple T2370888 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: DNA ligase Context triple: [Herbert Boyer, usedResearchTechnique, DNA ligase]
-
A.
DNA
DNA is a major social-democratic political party in Norway, historically one of the country’s dominant governing parties.
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B.
Szostak Design
Szostak Design is an architectural firm known for designing prominent cultural and performing arts venues, including the Durham Performing Arts Center in North Carolina.
-
C.
PCR
PCR was the abbreviation for the Romanian Communist Party, the ruling Marxist-Leninist political organization that governed Romania during most of the communist era in the 20th century.
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D.
Lextran
Lextran is the public bus transit system serving Lexington and surrounding areas in Fayette County, Kentucky.
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E.
TARDBP
TARDBP is a gene encoding the TDP-43 protein, a DNA/RNA-binding protein whose abnormal aggregation is a key pathological feature in amyotrophic lateral sclerosis and related neurodegenerative diseases.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: DNA ligase Target entity description: DNA ligase is an essential enzyme that joins breaks in the sugar-phosphate backbone of DNA, enabling processes such as DNA replication and genetic recombination.
-
A.
DNA
DNA is a major social-democratic political party in Norway, historically one of the country’s dominant governing parties.
-
B.
Szostak Design
Szostak Design is an architectural firm known for designing prominent cultural and performing arts venues, including the Durham Performing Arts Center in North Carolina.
-
C.
PCR
PCR was the abbreviation for the Romanian Communist Party, the ruling Marxist-Leninist political organization that governed Romania during most of the communist era in the 20th century.
-
D.
Lextran
Lextran is the public bus transit system serving Lexington and surrounding areas in Fayette County, Kentucky.
-
E.
TARDBP
TARDBP is a gene encoding the TDP-43 protein, a DNA/RNA-binding protein whose abnormal aggregation is a key pathological feature in amyotrophic lateral sclerosis and related neurodegenerative diseases.
- F. None of above. chosen
Statements (48)
| Predicate | Object |
|---|---|
| instanceOf |
DNA repair enzyme
ⓘ
enzyme ⓘ ligase ⓘ |
| actsOn | DNA sugar-phosphate backbone ⓘ |
| ATP-dependentFormTypicallyFoundIn |
archaea
ⓘ
eukaryotes ⓘ |
| belongsToEnzymeClass | EC 6 (ligases) ⓘ |
| biochemicalReactionType | ligation of DNA strand breaks ⓘ |
| biologicalRole |
DNA strand break sealing
ⓘ
prevention of DNA strand discontinuities ⓘ |
| catalyzes | formation of phosphodiester bonds in DNA ⓘ |
| discoveredInContextOf | DNA replication studies ⓘ |
| essentialFor |
completion of lagging strand synthesis
ⓘ
joining Okazaki fragments ⓘ maintenance of genome integrity ⓘ |
| formsBondBetween |
3'-hydroxyl group of one nucleotide
ⓘ
5'-phosphate group of adjacent nucleotide ⓘ |
| foundIn |
archaea
ⓘ
eukaryotes ⓘ prokaryotes ⓘ viruses ⓘ |
| hasECNumber | EC 6.5.1.- ⓘ |
| hasType |
ATP-dependent DNA ligase
ⓘ
NAD-dependent DNA ligase ⓘ |
| localizesTo |
mitochondria
ⓘ
nucleus ⓘ |
| mechanismStep |
formation of enzyme-AMP intermediate
ⓘ
nucleophilic attack by 3'-hydroxyl on activated 5'-phosphate ⓘ transfer of AMP to 5'-phosphate of DNA ⓘ |
| NAD-dependentFormTypicallyFoundIn | bacteria ⓘ |
| participatesIn |
DNA repair
ⓘ
DNA replication ⓘ base excision repair ⓘ genetic recombination ⓘ homologous recombination ⓘ mismatch repair ⓘ non-homologous end joining ⓘ nucleotide excision repair ⓘ |
| product | covalently continuous DNA strand ⓘ |
| requiresCofactor |
ATP
ⓘ
NAD+ ⓘ |
| substrate |
DNA with nicks
ⓘ
DNA with single-strand breaks ⓘ |
| usedIn |
construction of DNA libraries
ⓘ
ligation of DNA fragments into plasmid vectors ⓘ molecular cloning ⓘ next-generation sequencing library preparation ⓘ recombinant DNA technology ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: DNA ligase Description of subject: DNA ligase is an essential enzyme that joins breaks in the sugar-phosphate backbone of DNA, enabling processes such as DNA replication and genetic recombination.
Referenced by (2)
Full triples — surface form annotated when it differs from this entity's canonical label.