Sanger sequencing
E561076
Sanger sequencing is a DNA sequencing method that uses chain-terminating dideoxynucleotides to determine the precise order of nucleotides in a DNA molecule.
All labels observed (4)
| Label | Occurrences |
|---|---|
| Sanger sequencing canonical | 3 |
| BigDye Terminator sequencing chemistry | 1 |
| Sanger DNA sequencing | 1 |
| Sanger sequencing method | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T6004583 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: Sanger sequencing Context triple: [Frederick Sanger, knownFor, Sanger sequencing]
-
A.
Sanger
Sanger is a small city in California’s San Joaquin Valley known for its agricultural economy and proximity to Fresno.
-
B.
PCR
PCR was the abbreviation for the Romanian Communist Party, the ruling Marxist-Leninist political organization that governed Romania during most of the communist era in the 20th century.
-
C.
Edman
Edman is a masculine given name most notably borne by Edman Spangler, a carpenter implicated in the conspiracy surrounding U.S. President Abraham Lincoln’s assassination.
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D.
Edico Genome
Edico Genome was a biotechnology company specializing in high-speed genomic data analysis through its DRAGEN bio-IT platform and FPGA-based acceleration technology.
-
E.
Illumina
Illumina is a biotechnology company best known for developing and manufacturing advanced DNA sequencing and array-based technologies used in genomics research and clinical applications.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: Sanger sequencing Target entity description: Sanger sequencing is a DNA sequencing method that uses chain-terminating dideoxynucleotides to determine the precise order of nucleotides in a DNA molecule.
-
A.
Sanger
Sanger is a small city in California’s San Joaquin Valley known for its agricultural economy and proximity to Fresno.
-
B.
PCR
PCR was the abbreviation for the Romanian Communist Party, the ruling Marxist-Leninist political organization that governed Romania during most of the communist era in the 20th century.
-
C.
Edman
Edman is a masculine given name most notably borne by Edman Spangler, a carpenter implicated in the conspiracy surrounding U.S. President Abraham Lincoln’s assassination.
-
D.
Edico Genome
Edico Genome was a biotechnology company specializing in high-speed genomic data analysis through its DRAGEN bio-IT platform and FPGA-based acceleration technology.
-
E.
Illumina
Illumina is a biotechnology company best known for developing and manufacturing advanced DNA sequencing and array-based technologies used in genomics research and clinical applications.
- F. None of above. chosen
Statements (50)
| Predicate | Object |
|---|---|
| instanceOf |
DNA sequencing method
ⓘ
molecular biology technique ⓘ |
| alsoKnownAs |
chain-termination sequencing
ⓘ
dideoxy sequencing ⓘ |
| basedOn | DNA replication ⓘ |
| commonlyUsedFor |
clinical diagnostic sequencing of specific loci
ⓘ
sequencing individual genes ⓘ small-scale sequencing projects ⓘ validating variants detected by high-throughput sequencing ⓘ |
| contributedTo |
Human Genome Project
ⓘ
sequencing of the first complete genomes ⓘ |
| determines | nucleotide sequence of DNA ⓘ |
| developedBy | Frederick Sanger ⓘ |
| developedInYear | 1977 ⓘ |
| enabled | rapid sequencing of cloned DNA fragments ⓘ |
| errorTypesInclude |
base miscalls
ⓘ
uncertain base calls at low signal regions ⓘ |
| higherAccuracyThan | many next-generation sequencing methods per base ⓘ |
| limitationsInclude |
difficulty with long homopolymer regions
ⓘ
reduced quality near end of read ⓘ requirement for prior knowledge to design primers ⓘ |
| lowerThroughputThan | next-generation sequencing ⓘ |
| moreWidelyAdoptedThan | Maxam–Gilbert sequencing ⓘ |
| nowOftenUses |
capillary electrophoresis
ⓘ
fluorescently labeled dideoxynucleotides ⓘ |
| originallyAnalyzedBy | polyacrylamide gel electrophoresis ⓘ |
| originallyUsed |
four separate reactions
ⓘ
radioactively labeled nucleotides ⓘ |
| output | electropherogram ⓘ |
| produces | DNA fragments of varying length ⓘ |
| readLength | approximately 500–1000 bases ⓘ |
| reliesOn | selective incorporation of chain-terminating dideoxynucleotides ⓘ |
| requires |
annealing of primer
ⓘ
clonal DNA template ⓘ denaturation of DNA ⓘ extension by DNA polymerase ⓘ |
| templateCanBe |
M13 phage DNA
ⓘ
PCR product ⓘ plasmid DNA ⓘ |
| usedIn |
genotyping of specific loci
ⓘ
molecular cloning workflows ⓘ mutation detection ⓘ |
| uses |
DNA polymerase
ⓘ
DNA template ⓘ chain-terminating nucleotides ⓘ deoxynucleotides ⓘ dideoxynucleotides ⓘ primer ⓘ |
| wasKeyTo | first-generation DNA sequencing era ⓘ |
| wasPrecededBy | Maxam–Gilbert sequencing ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: Sanger sequencing Description of subject: Sanger sequencing is a DNA sequencing method that uses chain-terminating dideoxynucleotides to determine the precise order of nucleotides in a DNA molecule.
Referenced by (6)
Full triples — surface form annotated when it differs from this entity's canonical label.
subject linked to:
Sanger
linked to: Sanger sequencing
linked to: Sanger sequencing
linked to: Sanger sequencing