phiC31 integrase system
E459330
The phiC31 integrase system is a site-specific recombination tool widely used in genetic engineering to insert DNA precisely into defined genomic locations.
All labels observed (1)
| Label | Occurrences |
|---|---|
| phiC31 integrase system canonical | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T4645716 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: phiC31 integrase system Context triple: [Drosophila melanogaster, transgenesisMethod, phiC31 integrase system]
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A.
DNA ligase
DNA ligase is an essential enzyme that joins breaks in the sugar-phosphate backbone of DNA, enabling processes such as DNA replication and genetic recombination.
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B.
Phage Group
The Phage Group was an informal collective of mid-20th-century molecular biologists who used bacteriophages to uncover fundamental principles of genetics and helped lay the foundations of molecular biology.
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C.
A Genetic Switch: Phage Lambda and Higher Organisms
"A Genetic Switch: Phage Lambda and Higher Organisms" is a seminal molecular biology book that explains how gene regulation in bacteriophage lambda illuminates fundamental mechanisms of genetic control in more complex organisms.
-
D.
Szostak Design
Szostak Design is an architectural firm known for designing prominent cultural and performing arts venues, including the Durham Performing Arts Center in North Carolina.
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E.
CRISPR-Cas9 gene-editing technology
CRISPR-Cas9 gene-editing technology is a revolutionary molecular tool that allows scientists to precisely modify DNA within living organisms, transforming research, medicine, and biotechnology.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: phiC31 integrase system Target entity description: The phiC31 integrase system is a site-specific recombination tool widely used in genetic engineering to insert DNA precisely into defined genomic locations.
-
A.
DNA ligase
DNA ligase is an essential enzyme that joins breaks in the sugar-phosphate backbone of DNA, enabling processes such as DNA replication and genetic recombination.
-
B.
Phage Group
The Phage Group was an informal collective of mid-20th-century molecular biologists who used bacteriophages to uncover fundamental principles of genetics and helped lay the foundations of molecular biology.
-
C.
A Genetic Switch: Phage Lambda and Higher Organisms
"A Genetic Switch: Phage Lambda and Higher Organisms" is a seminal molecular biology book that explains how gene regulation in bacteriophage lambda illuminates fundamental mechanisms of genetic control in more complex organisms.
-
D.
Szostak Design
Szostak Design is an architectural firm known for designing prominent cultural and performing arts venues, including the Durham Performing Arts Center in North Carolina.
-
E.
CRISPR-Cas9 gene-editing technology
CRISPR-Cas9 gene-editing technology is a revolutionary molecular tool that allows scientists to precisely modify DNA within living organisms, transforming research, medicine, and biotechnology.
- F. None of above. chosen
Statements (47)
| Predicate | Object |
|---|---|
| instanceOf |
genetic engineering tool
ⓘ
site-specific recombination system ⓘ |
| derivedFrom | Streptomyces phage phiC31 ⓘ |
| developedFor | precise, predictable transgene insertion compared to random integration methods ⓘ |
| hasApplication |
chromosomal docking site strategies
ⓘ
functional genomics studies ⓘ gene therapy vector integration ⓘ generation of stable cell lines ⓘ genome engineering research ⓘ germline transgenesis in Drosophila melanogaster ⓘ germline transgenesis in mice ⓘ germline transgenesis in mosquitoes ⓘ germline transgenesis in rats ⓘ germline transgenesis in zebrafish ⓘ insertion of reporter constructs into defined loci ⓘ safe harbor locus targeting ⓘ site-specific integration in mammalian genomes ⓘ transgenesis in human cell lines ⓘ transgenesis in livestock ⓘ transgenesis in plants ⓘ |
| hasComponent |
attB recognition site
ⓘ
attP recognition site ⓘ phiC31 integrase enzyme ⓘ |
| hasEngineeringVariant |
codon-optimized phiC31 integrase for mammalian expression
ⓘ
mutant integrase variants with altered specificity ⓘ nuclear localization signal-tagged phiC31 integrase ⓘ |
| hasFunction |
enables precise DNA integration into defined genomic loci
ⓘ
facilitates stable genomic insertion of transgenes ⓘ mediates unidirectional site-specific recombination between attP and attB sites ⓘ |
| hasLimitation |
irreversibility of integration without recombination directionality factors
ⓘ
potential off-target integration at pseudo-attP or pseudo-attB sites in eukaryotic genomes ⓘ |
| hasProperty |
does not require host recombination factors
ⓘ
functions in a wide range of species ⓘ generates attL and attR hybrid sites after recombination ⓘ recognizes specific attB and attP sequences ⓘ unidirectional under normal conditions ⓘ |
| mechanism |
integrase binds attP and attB sites and catalyzes recombination
ⓘ
recombination produces attL and attR sites that are refractory to further integration ⓘ |
| requiresCofactor | divalent metal ions ⓘ |
| usedIn |
Drosophila attP docking site transgenic platforms
ⓘ
induced pluripotent stem cell engineering ⓘ mammalian safe harbor loci such as ROSA26 ⓘ stable integration of therapeutic genes in preclinical models ⓘ |
| usedWith |
expression cassettes for transgenes
ⓘ
genomic docking sites containing attP sequences ⓘ plasmid vectors carrying attB sites ⓘ |
| usesRecombinationType | serine recombinase-mediated recombination ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: phiC31 integrase system Description of subject: The phiC31 integrase system is a site-specific recombination tool widely used in genetic engineering to insert DNA precisely into defined genomic locations.
Referenced by (1)
Full triples — surface form annotated when it differs from this entity's canonical label.