Lowenstein–Jensen medium

E271796

Lowenstein–Jensen medium is a specialized egg-based solid growth medium used primarily for the isolation and cultivation of Mycobacterium species, especially Mycobacterium tuberculosis, in clinical microbiology.

All labels observed (2)

Label Occurrences
Lowenstein–Jensen medium canonical 9
Lowenstein-Jensen medium 1

How this entity was disambiguated

Statements (49)

Predicate Object
instanceOf culture medium ⓘ
egg-based medium ⓘ
selective medium ⓘ
solid growth medium ⓘ
alternativeTo Middlebrook 7H10 agar ⓘ
Middlebrook 7H11 agar ⓘ
classifiedAs biosafety level 3 culture medium when used with M. tuberculosis ⓘ
colonyMorphologyOnMedium rough, buff-colored colonies of Mycobacterium tuberculosis ⓘ
developedBy Ernst Lowenstein ⓘ
Kurt Jensen ⓘ
glycerolEffect enhances growth of Mycobacterium tuberculosis ⓘ
hasComponent asparagine ⓘ
disodium phosphate ⓘ
glycerol ⓘ
magnesium sulfate ⓘ
malachite green ⓘ
mineral salts ⓘ
monopotassium phosphate ⓘ
potato flour or starch ⓘ
whole eggs ⓘ
hasPreparationStep coagulation by inspissation ⓘ
dispensing into tubes or bottles ⓘ
mixing of mineral salt solution with eggs and other components ⓘ
hasProperty green color due to malachite green ⓘ
inhibits most contaminating bacteria ⓘ
selective for mycobacteria ⓘ
solidified by coagulation of eggs ⓘ
hasVariant Lowenstein–Jensen medium with pyruvate ⓘ
Lowenstein–Jensen medium without glycerol ⓘ
inhibitsGrowthOf most Gram-negative bacteria ⓘ
most Gram-positive bacteria ⓘ
pyruvateEffect enhances growth of Mycobacterium bovis ⓘ
qualityControlOrganism Mycobacterium tuberculosis H37Rv strain ⓘ
requiresIncubationAtmosphere aerobic conditions ⓘ
requiresIncubationTemperature 35–37 °C ⓘ
storageCondition refrigerated before use ⓘ
supportsGrowthOf Mycobacterium tuberculosis complex ⓘ
nontuberculous mycobacteria ⓘ
typicalIncubationTime up to 8 weeks for visible mycobacterial colonies ⓘ
usedFor cultivation of Mycobacterium species ⓘ
cultivation of Mycobacterium tuberculosis ⓘ
isolation of Mycobacterium species ⓘ
isolation of Mycobacterium tuberculosis ⓘ
usedIn clinical microbiology ⓘ
diagnosis of tuberculosis ⓘ
usedWithSpecimenType body fluids suspected of mycobacterial infection ⓘ
bronchoalveolar lavage ⓘ
sputum ⓘ
tissue biopsies ⓘ

How these facts were elicited

Referenced by (10)

Full triples — surface form annotated when it differs from this entity's canonical label.

Mycobacterium tuberculosis → cultureMedium → Lowenstein–Jensen medium ⓘ
Middlebrook 7H10 agar → comparedWith → Lowenstein–Jensen medium ⓘ
Mycobacterium bovis → growthMedium → Lowenstein–Jensen medium ⓘ
Mycobacterium marinum → growthMedium → Lowenstein–Jensen medium ⓘ
Middlebrook 7H11 agar → usedAlongWith → Lowenstein–Jensen medium ⓘ
Mycobacterium canettii → cultureMedium → Lowenstein-Jensen medium ⓘ
linked to: Lowenstein–Jensen medium
Kurt Jensen → coDeveloperOf → Lowenstein–Jensen medium ⓘ
Kurt Jensen → notableWork → Lowenstein–Jensen medium ⓘ
Ernst Lowenstein → notableWork → Lowenstein–Jensen medium ⓘ
Mycobacterium kansasii → cultureMedium → Lowenstein–Jensen medium ⓘ